Generator
Part:BBa_K3431050:Design
Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang Group: iGEM20_CSMU_Taiwan (2020-10-17)
Banana_ToeholdSwitch-Regulated sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 865
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We included T7 promoter and terminator to ensure correct functioning of this device in PURExpress in vitro protein synthesis kit.
Source
We obtained the sequence of sfGFP from BBa_K3431031, and synthesized the construct through IDT.