Generator

Part:BBa_K3431050:Design

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-10-17)


Banana_ToeholdSwitch-Regulated sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 865
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We included T7 promoter and terminator to ensure correct functioning of this device in PURExpress in vitro protein synthesis kit.


Source

We obtained the sequence of sfGFP from BBa_K3431031, and synthesized the construct through IDT.

References